5UXE
Crystal Structure of Inosine 5'-monophosphate Dehydrogenase from Clostridium perfringens Complexed with IMP and P178
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.937, 119.101, 96.971 |
| Unit cell angles | 90.00, 102.86, 90.00 |
Refinement procedure
| Resolution | 39.253 - 2.100 |
| R-factor | 0.1757 |
| Rwork | 0.173 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q32 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.919 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 80071 | 3680 |
| <I/σ(I)> | 11.14 | |
| Completeness [%] | 94.4 | 88.1 |
| Redundancy | 2.7 | 2.2 |
| CC(1/2) | 0.625 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2M ammonium acetate, 0.1M Bis-Tris pH 6.5, 45% MPD |
