5UXB

Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium, apoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-02-13
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	75.933, 79.741, 99.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.753 - 2.794
R-factor	0.2208
Rwork	0.219
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uxc
RMSD bond length	0.003
RMSD bond angle	0.565
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2733)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.800	2.800
Rpim	0.048	0.200
Number of reflections	25067	771
<I/σ(I)>	16.28	3.78
Completeness [%]	99.9	100
Redundancy	9.6	9.5
CC(1/2)		0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	2 M ammonium sulfate, 2% PEG 400, 0.1 M HEPES pH 7.5, 1% trehalose