5UWX
Crystal Structure of Inosine 5'-monophosphate Dehydrogenase from Clostridium perfringens Complexed with IMP and P176
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.278, 118.943, 96.992 |
| Unit cell angles | 90.00, 102.88, 90.00 |
Refinement procedure
| Resolution | 47.500 - 1.850 |
| R-factor | 0.1642 |
| Rwork | 0.163 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.021 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 118158 | 5833 |
| <I/σ(I)> | 16.34 | 1.79 |
| Completeness [%] | 97.5 | 96.7 |
| Redundancy | 5.9 | 5.7 |
| CC(1/2) | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2M ammonium acetate, 0.1M Bis-Tris pH 6.5, 45% MPD |
