5UWJ

Crystal Structure of FMRP NES Peptide in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2015-02-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.493, 106.493, 303.928
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.625 - 2.221
R-factor	0.181
Rwork	0.180
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hb2
RMSD bond length	0.004
RMSD bond angle	0.631
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.220	2.220
Rmerge	0.077	0.966
Rpim	0.033	0.408
Number of reflections	86622	4266
<I/σ(I)>	21.6	2.1
Completeness [%]	99.4	100
Redundancy	6.2
CC(1/2)		0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	295	17% PEG3350, 100 mM Bis-Tris, pH 6.4, 200 mM ammonium nitrate, 10 mM spermine-HCl, 4 mM HCl