5UVT

BRD4_BD2_A-1454056

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	63.497, 70.407, 33.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.200 - 1.670
R-factor	0.168
Rwork	0.167
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Apo BRD4_BD2
RMSD bond length	0.010
RMSD bond angle	0.920
Data reduction software	XDS (2.11.7)
Data scaling software	SCALA
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.200
High resolution limit [Å]	1.670
Rmerge	0.070
Number of reflections	18056
<I/σ(I)>	18.9
Completeness [%]	100.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Protein Buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0