5UV8

Interleukin-3 Receptor Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	P 65 2 2
Unit cell lengths	132.039, 132.039, 210.631
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	114.350 - 2.700
R-factor	0.24404
Rwork	0.242
R-free	0.28564
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jzj 1jli
RMSD bond length	0.008
RMSD bond angle	1.405
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	114.350	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.170	0.740
Number of reflections	30501
<I/σ(I)>	25.7
Completeness [%]	100.0	100
Redundancy	8.1	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 8000, 200 mM NaCI, 100 mM citrate-phosphate buffer pH 5