5UTK

Crystal structure of the membrane proximal three fibronectin type III (FNIII) domains of Tie2 (Tie2[FNIIIa-c])

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-18
Detector	MARRESEARCH
Wavelength(s)	1.04, 0.97944, 0.97927, 0.94928
Spacegroup name	C 1 2 1
Unit cell lengths	174.950, 52.026, 111.427
Unit cell angles	90.00, 117.49, 90.00

Refinement procedure

Resolution	49.328 - 2.500
R-factor	0.2266
Rwork	0.225
R-free	0.24720
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.901
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.570
High resolution limit [Å]	2.500	5.720	2.500
Rmerge	0.106	0.065	0.562
Total number of observations	95446
Number of reflections	30201
<I/σ(I)>	9.2
Completeness [%]	97.4	97.4	85.1
Redundancy	3.2	3.2	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	25-40% glycerol, 2.5-15% PEG 3K, 100 mM sodium acetate, 100 mM sodium phosphate, pH 4.5