5UTE
Kaposi's Sarcoma Herpesvirus Protease in Complex with Allosteric Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 68.330, 93.328, 119.147 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.472 - 2.050 |
R-factor | 0.1855 |
Rwork | 0.183 |
R-free | 0.23590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3njq |
RMSD bond length | 0.014 |
RMSD bond angle | 1.447 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHENIX (Phenix 1.8.4 Phaser 2.1) |
Refinement software | PHENIX (1.10pre_2100) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 73.470 |
High resolution limit [Å] | 2.050 |
Rmerge | 0.374 |
Number of reflections | 46042 |
<I/σ(I)> | 8.97 |
Completeness [%] | 100.0 |
Redundancy | 15.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 0.2M Imidazole pH 8.0, 0.4M NaH2PO4/1.6M K2HPO4, 0.2M NaCl |