5USA
5-Se-T2-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.201, 37.731, 62.238 |
| Unit cell angles | 90.00, 96.38, 90.00 |
Refinement procedure
| Resolution | 35.656 - 1.800 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21642 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.905 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Rpim | 0.025 |
| Number of reflections | 17472 |
| <I/σ(I)> | 29 |
| Completeness [%] | 100.0 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M MES pH 6.5, 12%(w/v) PEG 20000 |
