5URZ

Crystal structure of Frizzled 5 CRD in complex with BOG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	93
Detector technology	CCD
Collection date	2012-09-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.99999
Spacegroup name	P 31 2 1
Unit cell lengths	123.051, 123.051, 46.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.556 - 2.202
R-factor	0.1818
Rwork	0.180
R-free	0.22360
RMSD bond length	0.011
RMSD bond angle	1.215
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	20901
<I/σ(I)>	25.4
Completeness [%]	99.9
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1 M sodium citrate tribasic dehydrate pH 5.5, 22% polyethylene glycol 3350