5URY
Crystal structure of Frizzled 5 CRD in complex with PAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2012-10-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 123.412, 123.412, 46.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.986 - 2.098 |
| R-factor | 0.1722 |
| Rwork | 0.170 |
| R-free | 0.20650 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.176 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.098 |
| Number of reflections | 24306 |
| <I/σ(I)> | 28.1 |
| Completeness [%] | 100.0 |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1 M sodium citrate tribasic dehydrate pH 5.5, 22% polyethylene glycol 3350 |
