5UQE
Multidomain structure of human kidney-type glutaminase(KGA/GLS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 144.420, 144.420, 615.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.642 - 3.600 |
R-factor | 0.2762 |
Rwork | 0.274 |
R-free | 0.31270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.566 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.29) |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2733) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.642 | 49.640 | 3.670 |
High resolution limit [Å] | 3.600 | 18.000 | 3.600 |
Rmerge | 0.349 | 0.067 | 2.214 |
Rmeas | 0.357 | 0.068 | 2.269 |
Rpim | 0.074 | 0.015 | 0.494 |
Number of reflections | 143726 | ||
<I/σ(I)> | 8.8 | ||
Completeness [%] | 100.0 | 95.7 | 100 |
Redundancy | 23.4 | 19.8 | 20.8 |
CC(1/2) | 0.998 | 0.995 | 0.716 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 277 | BIS-TRIS propane 0.1 M pH 6,8; Sodium Formate 1.8 M; Sodium Chloride 0.5 M; BPTES 1.2 mM |