5UPL
CDC42 binds PAK4 via an extended GTPase-effector inteface - 2 peptide: PAK4FL, CDC42 - UNREFINED
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97922 |
Spacegroup name | P 63 |
Unit cell lengths | 141.652, 141.652, 62.093 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.637 - 3.003 |
R-factor | 0.2593 |
Rwork | 0.257 |
R-free | 0.31020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fie |
RMSD bond length | 0.023 |
RMSD bond angle | 1.617 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.637 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.117 | |
Rpim | 0.034 | 0.707 |
Number of reflections | 14433 | |
<I/σ(I)> | 20.3 | 1.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 18 | |
CC(1/2) | 0.588 | 0.588 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1M Tris-HCl pH 8.5, 50mM Na2SO4, 6% PEG6000 |