5UPH

Lipids bound lysosomal integral membrane protein 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 42 21 2
Unit cell lengths	139.040, 139.040, 178.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.610 - 3.000
R-factor	0.195
Rwork	0.193
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q4f
RMSD bond length	0.010
RMSD bond angle	1.280
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.310	4.240
High resolution limit [Å]	3.000	3.000
Rmerge	0.151	0.631
Rpim		0.266
Number of reflections	35701	22726
<I/σ(I)>	12.4	4
Completeness [%]	99.9	99.9
Redundancy	12.4	12.3
CC(1/2)	0.999	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	protein in 10 mM HEPES, pH 7.5 200 mM NaCl mixed with equal amount of 0.22 M NaCl, 29% PPG 400