5UPH
Lipids bound lysosomal integral membrane protein 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 139.040, 139.040, 178.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.610 - 3.000 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q4f |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.310 | 4.240 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.151 | 0.631 |
| Rpim | 0.266 | |
| Number of reflections | 35701 | 22726 |
| <I/σ(I)> | 12.4 | 4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12.4 | 12.3 |
| CC(1/2) | 0.999 | 0.981 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | protein in 10 mM HEPES, pH 7.5 200 mM NaCl mixed with equal amount of 0.22 M NaCl, 29% PPG 400 |
