5UPD
Methyltransferase domain of human Wolf-Hirschhorn Syndrome Candidate 1-Like protein 1 (WHSC1L1)
Replaces: 4YZ8Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-29 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97957 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.160, 62.810, 48.840 |
Unit cell angles | 90.00, 109.78, 90.00 |
Refinement procedure
Resolution | 41.550 - 1.800 |
R-factor | 0.1856 |
Rwork | 0.184 |
R-free | 0.21820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ooi |
RMSD bond length | 0.016 |
RMSD bond angle | 1.801 |
Data reduction software | xia2 |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.550 | 41.550 | 1.850 |
High resolution limit [Å] | 1.800 | 8.060 | 1.800 |
Rmerge | 0.105 | 0.038 | 0.714 |
Rmeas | 0.124 | 0.045 | 0.859 |
Rpim | 0.064 | 0.024 | 0.471 |
Total number of observations | 85056 | 912 | 5383 |
Number of reflections | 23067 | ||
<I/σ(I)> | 11.1 | 31.8 | 2 |
Completeness [%] | 98.9 | 94.7 | 96.2 |
Redundancy | 3.7 | 3.4 | 3.2 |
CC(1/2) | 0.995 | 0.997 | 0.626 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG-5000-MME, 0.1 M bis-tris, 0.02 M sarcosine |