5UPD

Methyltransferase domain of human Wolf-Hirschhorn Syndrome Candidate 1-Like protein 1 (WHSC1L1)

Replaces:  4YZ8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-29
Detector	RAYONIX MX-300
Wavelength(s)	0.97957
Spacegroup name	P 1 21 1
Unit cell lengths	44.160, 62.810, 48.840
Unit cell angles	90.00, 109.78, 90.00

Refinement procedure

Resolution	41.550 - 1.800
R-factor	0.1856
Rwork	0.184
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ooi
RMSD bond length	0.016
RMSD bond angle	1.801
Data reduction software	xia2
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.550	41.550	1.850
High resolution limit [Å]	1.800	8.060	1.800
Rmerge	0.105	0.038	0.714
Rmeas	0.124	0.045	0.859
Rpim	0.064	0.024	0.471
Total number of observations	85056	912	5383
Number of reflections	23067
<I/σ(I)>	11.1	31.8	2
Completeness [%]	98.9	94.7	96.2
Redundancy	3.7	3.4	3.2
CC(1/2)	0.995	0.997	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	20% PEG-5000-MME, 0.1 M bis-tris, 0.02 M sarcosine