5UOT
CryoEM structure of the helical assembly of full length MxB
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.2 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.6 |
| Number of Particle | 51553 |
| Software | EMAN,RELION,NAMD,Rosetta,SerialEM,Gctf,MDFF |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
