5UMU
Crystal structure of the middle double PH domain of human FACT complex subunit SPT16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-03-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.221, 99.819, 120.314 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.100 - 1.903 |
R-factor | 0.1581 |
Rwork | 0.157 |
R-free | 0.19110 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.688 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.096 | 0.613 |
Number of reflections | 91465 | 4677 |
<I/σ(I)> | 23.8 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 10.4 | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 2% Tacsimate pH 5.0, 100 mM Sodium citrate tribasic dihydrate pH 5.6, 10-20% Polyethylene glycol 3350 |