5UM8
Crystal structure of HIV-1 envelope trimer 16055 NFL TD CC (T569G) in complex with Fabs 35022 and PGT124
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 63 |
Unit cell lengths | 126.456, 126.456, 314.093 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.247 - 3.935 |
R-factor | 0.2634 |
Rwork | 0.260 |
R-free | 0.32850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cez 4r26 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.640 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.250 | 4.020 |
High resolution limit [Å] | 3.935 | 3.935 |
Rmerge | 0.194 | 1.510 |
Rmeas | 0.217 | 1.690 |
Rpim | 0.096 | 0.750 |
Number of reflections | 25153 | 1224 |
<I/σ(I)> | 7.5 | 1.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5 | 4.9 |
CC(1/2) | 0.923 | 0.640 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.03 | 277 | 0.2M ammonium sulfate, 10% glycerol, 22% PEG 300, 0.1M phosphate-citrate |