5ULK
Crystal Structure of RNF165 in complex with a UbcH5b~Ub conjugate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.109, 73.714, 121.559 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.780 - 2.380 |
R-factor | 0.2096 |
Rwork | 0.206 |
R-free | 0.26730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.484 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.779 | 31.515 | 2.510 |
High resolution limit [Å] | 2.381 | 7.530 | 2.380 |
Rmerge | 0.042 | 0.734 | |
Rmeas | 0.074 | 0.046 | 0.807 |
Rpim | 0.028 | 0.018 | 0.325 |
Total number of observations | 85553 | 2531 | 8615 |
Number of reflections | 12481 | ||
<I/σ(I)> | 13.3 | 34 | 1.9 |
Completeness [%] | 97.5 | 91.5 | 85.2 |
Redundancy | 6.9 | 6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 200 mM ammonium nitrate, 20% PEG 3350 |