5UKB

VSV N PROTEIN IN COMPLEX WITH INHIBITORY NANOBODY 1004

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 2
Unit cell lengths	240.117, 335.497, 75.899
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	137.515 - 5.473
R-factor	0.3365
Rwork	0.336
R-free	0.33750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uk4 4krl
RMSD bond length	0.004
RMSD bond angle	0.851
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	195.260	5.700
High resolution limit [Å]	5.470	5.470
Rpim	0.052	0.460
Number of reflections	20655
<I/σ(I)>	16.2	0.86
Completeness [%]	99.7	98.3
Redundancy	11	5.5
CC(1/2)	0.990	0.556

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	0.1 M sodium acetate pH 5.0 1.5 M ammonium sulfate