Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-01 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 126.902, 171.950, 135.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.259 - 1.900 |
| R-factor | 0.1764 |
| Rwork | 0.175 |
| R-free | 0.20950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ma9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.254 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.100 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 225895 |
| <I/σ(I)> | 13.2 |
| Completeness [%] | 100.0 |
| Redundancy | 19.6 |
| CC(1/2) | 0.200 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 1.4 MgSO4, 0.1 M MES pH 6.5. |
