5UK4

VESICULAR STOMATITS VIRUS N PROTEIN IN COMPLEX WITH INHIBITORY NANOBODY 1307

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 1
Unit cell lengths	147.555, 156.008, 217.450
Unit cell angles	79.24, 75.66, 62.27

Refinement procedure

Resolution	128.352 - 3.204
R-factor	0.237
Rwork	0.236
R-free	0.28770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2GIC (VSV N) and 5TJW (VHH)
RMSD bond length	0.002
RMSD bond angle	0.525
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	128.450	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.200	0.675
Rpim	0.152	0.502
Number of reflections	250547
<I/σ(I)>	5.1	1.4
Completeness [%]	92.4	92.5
Redundancy	2.5	2.4
CC(1/2)	0.990	0.619

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.2 M sodium acetate, 0.1 M tri-sodium citrate pH 5.5, 5% (w/v) polyethylene glycol 4000