5UJC

Crystal structure of a C.elegans B12-trafficking protein CblC, a human MMACHC homologue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03325
Spacegroup name	P 21 21 21
Unit cell lengths	53.008, 66.089, 92.826
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.840 - 1.350
R-factor	0.13361
Rwork	0.132
R-free	0.16303
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sbz
RMSD bond length	0.015
RMSD bond angle	2.013
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.840	1.370
High resolution limit [Å]	1.350	1.350
Rmerge	0.054	0.573
Rpim	0.029	0.435
Number of reflections	66799	2029
<I/σ(I)>	15	1.9
Completeness [%]	92.9	58.7
Redundancy	3.8	2.3
CC(1/2)	0.997	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	21.5% PEG 3350, 40 mM Spermidine-HCl, 0.2 M (NH4)2tartarate