5UJ7
Structure of the active form of human Origin Recognition Complex ATPase motor module, complex subunitS 1, 4, 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.887, 81.141, 151.947 |
| Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
| Resolution | 19.882 - 3.394 |
| R-factor | 0.245 |
| Rwork | 0.242 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xgc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.718 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.520 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Number of reflections | 39700 | |
| <I/σ(I)> | 8.75 | 1.2 |
| Completeness [%] | 97.0 | 96 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 22% Ethylene glycol 8% Propylene glycol 10mg/ml protein 1:1 ratio |
