5UHV

wild-type NRas bound to GppNHp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	291.15
Detector technology	IMAGE PLATE
Collection date	2014-07-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	39.007, 39.007, 159.175
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.496 - 1.672
R-factor	0.1909
Rwork	0.187
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k8y
RMSD bond length	0.007
RMSD bond angle	0.913
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	28.500
High resolution limit [Å]	1.670
Rmerge	0.079
Number of reflections	16930
<I/σ(I)>	24
Completeness [%]	98.5
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trihydrate pH 6.5, 20% w/v PEG 8000 (Hampton Research Crystal Screen number 18)