5UHM
Apo-Structure of Mature Growth Differentiation Factor 11
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-12-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.10537 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 65.160, 65.160, 101.942 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.371 - 1.900 |
R-factor | 0.2186 |
Rwork | 0.217 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e4g |
RMSD bond length | 0.007 |
RMSD bond angle | 0.853 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.970 | 50.970 | 1.940 |
High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
Rmerge | 0.069 | 0.041 | 0.706 |
Rmeas | 0.077 | 0.047 | 0.775 |
Rpim | 0.032 | 0.021 | 0.316 |
Number of reflections | 20340 | ||
<I/σ(I)> | 10.4 | ||
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 5.8 | 5.2 | 5.9 |
CC(1/2) | 0.996 | 0.996 | 0.963 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.75 | 298.15 | 5mM Calcium Chloride 0.1M Sodium Acetate 33% MPD |