5UHA
Crystal structure of Mycobacterium tuberculosis transcription initiation complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.925 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 150.197, 163.481, 195.653 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.913 - 3.906 |
R-factor | 0.2149 |
Rwork | 0.211 |
R-free | 0.27910 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.040 |
High resolution limit [Å] | 3.900 | 8.390 | 3.900 |
Rmerge | 0.219 | 0.110 | |
Rmeas | 0.226 | 0.113 | |
Rpim | 0.057 | 0.025 | 0.387 |
Total number of observations | 659212 | ||
Number of reflections | 44080 | ||
<I/σ(I)> | 3.6 | ||
Completeness [%] | 99.2 | 99 | 99.4 |
Redundancy | 15 | 20 | 8.2 |
CC(1/2) | 0.994 | 0.144 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 295 | 50 mM Tris-HCl, pH 8.2, 200 mM KCl, 50 mM MgCl2, and 10% PEG3350 |