5UGP
DNA polymerase beta complex with a 1nt gap and dCMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-08 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.784, 79.966, 55.454 |
| Unit cell angles | 90.00, 107.68, 90.00 |
Refinement procedure
| Resolution | 24.629 - 1.955 |
| R-factor | 0.1851 |
| Rwork | 0.182 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.894 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.629 | 1.990 |
| High resolution limit [Å] | 1.955 | 1.955 |
| Rmerge | 0.070 | 0.430 |
| Number of reflections | 30285 | |
| <I/σ(I)> | 18.9 | 2.4 |
| Completeness [%] | 99.3 | 93.5 |
| Redundancy | 4.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350 |
