5UGL

Crystal Structure of FGF Receptor 2 Tyrosine Kinase Domain Harboring the D650V Activating Mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97912
Spacegroup name	P 21 21 21
Unit cell lengths	67.215, 78.786, 116.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.843 - 1.861
R-factor	0.2207
Rwork	0.219
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2psq
RMSD bond length	0.007
RMSD bond angle	0.882
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.860	1.860
Number of reflections	46452
<I/σ(I)>	75.5	17.3
Completeness [%]	87.9	57.6
Redundancy	14	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25 mM HEPES pH7.5, 15%-25% w/v PEG4000, 0.2-0.3 M NH4SO3