5UFH
The crystal structure of a LacI-type transcription regulator from Bifidobacterium animalis subsp. lactis DSM 10140
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.326, 60.171, 67.188 |
| Unit cell angles | 90.00, 109.22, 90.00 |
Refinement procedure
| Resolution | 22.574 - 1.450 |
| R-factor | 0.1657 |
| Rwork | 0.165 |
| R-free | 0.18430 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.574 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.088 | 0.699 |
| Number of reflections | 46714 | |
| <I/σ(I)> | 38.9 | 2.1 |
| Completeness [%] | 96.8 | 93.8 |
| Redundancy | 4.9 | 4.3 |
| CC(1/2) | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris:HCl, 6 M Ammonium Nitrate |
