5UFE

Wild-type K-Ras(GNP)/R11.1.6 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-05-13
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	48.713, 64.648, 74.506
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.772 - 2.302
R-factor	0.1901
Rwork	0.184
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 3GFT and PDB ID 1SSO
RMSD bond length	0.008
RMSD bond angle	1.056
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.770	2.384
High resolution limit [Å]	2.302	2.302
Rmerge	0.129	0.305
Number of reflections	10700
<I/σ(I)>	17.31	6.05
Completeness [%]	98.0	97.75
Redundancy	6.7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	calcium chloride, cadmium chloride, cobalt(II)chloride, PEG 3350