5UFE
Wild-type K-Ras(GNP)/R11.1.6 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-05-13 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.713, 64.648, 74.506 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.772 - 2.302 |
R-factor | 0.1901 |
Rwork | 0.184 |
R-free | 0.24120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 3GFT and PDB ID 1SSO |
RMSD bond length | 0.008 |
RMSD bond angle | 1.056 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.770 | 2.384 |
High resolution limit [Å] | 2.302 | 2.302 |
Rmerge | 0.129 | 0.305 |
Number of reflections | 10700 | |
<I/σ(I)> | 17.31 | 6.05 |
Completeness [%] | 98.0 | 97.75 |
Redundancy | 6.7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | calcium chloride, cadmium chloride, cobalt(II)chloride, PEG 3350 |