5UDJ
IFIT1 monomeric mutant (L457E/L464E) with Gppp-AAAA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 94 |
Detector technology | CCD |
Collection date | 2016-01-16 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.979 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 112.536, 112.536, 93.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.299 - 1.690 |
R-factor | 0.1602 |
Rwork | 0.159 |
R-free | 0.18100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.283 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2142) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.720 |
High resolution limit [Å] | 1.690 | 4.590 | 1.690 |
Rmerge | 0.122 | 0.061 | |
Number of reflections | 67695 | ||
<I/σ(I)> | 22.2 | 1.9 | |
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 14.5 | 13.8 | 12.2 |
CC(1/2) | 0.998 | 0.704 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 277.15 | 27 - 32 % PEG 200, 0.1 M Tris pH 8.1, 200 mM CaCl2 |