5UDH

HHARI/ARIH1-UBCH7~Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	C 1 2 1
Unit cell lengths	184.571, 76.788, 147.724
Unit cell angles	90.00, 107.33, 90.00

Refinement procedure

Resolution	141.010 - 3.240
R-factor	0.22493
Rwork	0.222
R-free	0.27906
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.382
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CRANK
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	141.010	3.450
High resolution limit [Å]	3.240	3.240
Rmerge	0.093	0.883
Number of reflections	31387
<I/σ(I)>	13.1	1.9
Completeness [%]	98.9	96
Redundancy	5.8	5.9
CC(1/2)	1.000	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	1 ul protein mixture with 10-12 mg/ml protein in 25 mM Tris pH 7.0, 150 mM NaCl, 1 mM DTT and 1 ul well solution with 7-10% PEG 5000 MME, 0.1 M HP pH 7.0 and 5% Tascimate pH 7.0. Over 1 ml reservoir.