5UDC
Crystal Structure of RSV F A2 Bound to MEDI8897
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.780, 126.310, 162.310 |
| Unit cell angles | 90.00, 102.56, 90.00 |
Refinement procedure
| Resolution | 43.139 - 3.450 |
| R-factor | 0.1775 |
| Rwork | 0.175 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jhw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.961 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.140 | 43.140 | 3.560 |
| High resolution limit [Å] | 3.450 | 14.220 | 3.450 |
| Rmerge | 0.085 | 0.034 | 0.565 |
| Rmeas | 0.102 | ||
| Rpim | 0.056 | ||
| Total number of observations | 165678 | ||
| Number of reflections | 53451 | ||
| <I/σ(I)> | 8 | ||
| Completeness [%] | 99.0 | 89.2 | 99.7 |
| Redundancy | 3.1 | 2.9 | 3.1 |
| CC(1/2) | 0.996 | 0.997 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 10% w/v PEG8000, 20% v/v ethylene glycol, 0.1 M Tris/BICINE pH 8.5, 0.02 M carboxylic acids, 0.3 M NDSB-195 |
