5UD6
Crystal structure of DHDPS from Cyanidioschyzon merolae with lysine bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-03-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.630, 74.700, 97.550 |
| Unit cell angles | 90.00, 103.31, 90.00 |
Refinement procedure
| Resolution | 94.930 - 2.400 |
| R-factor | 0.2142 |
| Rwork | 0.212 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.661 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.1) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.930 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.207 | 1.161 |
| Total number of observations | 309893 | 33668 |
| Number of reflections | 41728 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 93.2 | 96 |
| Redundancy | 7.4 | 7.5 |
| CC(1/2) | 0.993 | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M CaCl2 and 20% w/v PEG3350 |
