5UD5
Crystal structure of the tRNA binding domain of Pyrrolysyl-tRNA synthetase bound to tRNA(Pyl)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-17 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979200 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 72.280, 72.280, 238.970 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.218 - 2.347 |
R-factor | 0.2191 |
Rwork | 0.216 |
R-free | 0.24230 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.835 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.220 |
High resolution limit [Å] | 2.347 |
Number of reflections | 31207 |
<I/σ(I)> | 22.57 |
Completeness [%] | 99.7 |
Redundancy | 9.79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | HEPES, magnesium chloride, PEG3350 |