5UCT

Mycobacterium tuberculosis toxin MazF-mt6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 64 2 2
Unit cell lengths	105.220, 105.220, 144.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.433 - 2.700
R-factor	0.2401
Rwork	0.239
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mdx
RMSD bond length	0.003
RMSD bond angle	0.550
Data reduction software	DIALS
Data scaling software	Aimless
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.000
High resolution limit [Å]	2.700
Number of reflections	13353
<I/σ(I)>	12
Completeness [%]	98.0
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	50 mM sodium cacodylate pH 6.0, 10 mM magnesium chloride, 1.05 M lithium sulfate