5UCI
Hsp90b N-terminal domain with inhibitors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-02 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 65 |
| Unit cell lengths | 129.273, 129.273, 107.327 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.227 - 2.700 |
| R-factor | 0.1849 |
| Rwork | 0.181 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uym |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.043 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 28083 | 2785 |
| <I/σ(I)> | 17.9 | 3.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 30% PEG 8,000, 0.2 M sodium acetate, 0.1 sodium cacodylate pH 6.5 |
