5UBZ

Fab structure of HIV gp120 specific mAb 1E12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033200
Spacegroup name	P 43 21 2
Unit cell lengths	79.174, 79.174, 124.377
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.608 - 2.750
R-factor	0.1931
Rwork	0.189
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wuu
RMSD bond length	0.009
RMSD bond angle	1.167
Data reduction software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.610	2.920
High resolution limit [Å]	2.750	2.750
Number of reflections	19611
<I/σ(I)>	16.61	2.01
Completeness [%]	99.8	99.8
Redundancy	6.17	6.09
CC(1/2)	0.999	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291.15	28% (w/v) PEG 4000, 0.085 M sodium Acetate pH 4.6, and 15% glycerol