5UAR

Dephosphorylated, ATP-free cystic fibrosis transmembrane conductance regulator (CFTR) from zebrafish

Replaces:  5TSI

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	0.01
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.73
Number of Particle	803894
Software	RELION,FREALIGN,REFMAC,SerialEM,CTFFIND4,UCSF Chimera
Symmetry	C1
CTF correction	NONE