5UAO
Crystal structure of MibH, a lathipeptide tryptophan 5-halogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.436, 87.887, 100.632 |
| Unit cell angles | 64.58, 69.90, 76.26 |
Refinement procedure
| Resolution | 87.380 - 1.880 |
| R-factor | 0.15855 |
| Rwork | 0.156 |
| R-free | 0.20362 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wet |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.942 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 87.380 |
| High resolution limit [Å] | 1.880 |
| Number of reflections | 170761 |
| <I/σ(I)> | 16.8 |
| Completeness [%] | 97.8 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 12.5% MPD, 12.5% PEG 3.35K, 0.1 M MES/imidazole pH 6.5, and 0.09 M NPS (1:1:1 NaNO3, Na2HPO4, (NH4)2SO4) |
