5UAB
MET Tyrosine Kinase Inhibition Enhances the Antitumor Efficacy of an HGF Antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-29 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9764848 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.333, 47.167, 159.821 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.1966 |
| Rwork | 0.195 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.137 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 4.350 | 1.900 |
| Rmerge | 0.093 | 0.099 | 0.282 |
| Total number of observations | 154062 | ||
| Number of reflections | 24319 | ||
| <I/σ(I)> | 11.1 | ||
| Completeness [%] | 92.8 | 99 | 65.9 |
| Redundancy | 6.3 | 6.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 16.% PEG MME 5000, 15.% Isopropanol, 0.1M HEPES pH 7.8 |
