5U8W
Dihydrolipoamide dehydrogenase (LpdG) from Pseudomonas aeruginosa bound to NADH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.510, 117.830, 139.730 |
Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
Resolution | 34.935 - 1.790 |
R-factor | 0.1668 |
Rwork | 0.167 |
R-free | 0.17850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lpf |
RMSD bond length | 0.004 |
RMSD bond angle | 0.745 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.280 | 1.850 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.085 | 1.410 |
Number of reflections | 198646 | |
<I/σ(I)> | 17.3 | 2.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.5 | 13.8 |
CC(1/2) | 0.999 | 0.793 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | PEG 3350, HEPES, KCl, microseeding |