5U8U

Dihydrolipoamide dehydrogenase (LpdG) from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	65.320, 116.280, 136.590
Unit cell angles	90.00, 94.73, 90.00

Refinement procedure

Resolution	38.755 - 1.350
R-factor	0.1328
Rwork	0.133
R-free	0.15650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lpf
RMSD bond length	0.012
RMSD bond angle	1.213
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.760	1.400
High resolution limit [Å]	1.350	1.350
Rmerge	0.062	1.736
Number of reflections	443473
<I/σ(I)>	19.3	1.4
Completeness [%]	99.9	99.5
Redundancy	13.6	13.4
CC(1/2)	1.000	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293.15	PEG 3350, HEPES, KCl, microseeding