5U7A
Crystal structure of a complex formed between MerB and Dimethyltin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.055, 88.648, 51.490 |
| Unit cell angles | 90.00, 100.34, 90.00 |
Refinement procedure
| Resolution | 43.980 - 1.532 |
| R-factor | 0.1748 |
| Rwork | 0.173 |
| R-free | 0.21050 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.346 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.530 |
| Number of reflections | 43607 |
| <I/σ(I)> | 16.45 |
| Completeness [%] | 86.5 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | 23 % polyethylene glycol 2000 MME, 0.2 M sodium acetate pH 5.5, 0.2 M potassium bromide. Before flash freezing, the same precipitant was used except 25% polyethylene glycol 2000 MME was used as a cryo-protectant. |
