5U77
Crystal structure of ORP8 PH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 57.079, 57.079, 154.949 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.712 - 2.157 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v88 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.443 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.712 | 2.270 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.100 | |
| Number of reflections | 7941 | |
| <I/σ(I)> | 18.4 | |
| Completeness [%] | 91.0 | |
| Redundancy | 16.9 | |
| CC(1/2) | 0.999 | 0.594 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES, pH 7.5, 0.2 M Ammonium formate, 0.2 M NDSB-195, 27% PEG 3350 |
