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5U68

Structural basis for antibody cross-neutralization of respiratory syncytial virus and human metapneumovirus

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-F
Synchrotron siteAPS
Beamline21-ID-F
Temperature [K]100
Detector technologyCCD
Collection date2015-08-13
DetectorRAYONIX MX-225
Wavelength(s)0.97872
Spacegroup nameP 31 2 1
Unit cell lengths155.540, 155.540, 275.930
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution48.191 - 3.083
R-factor0.187
Rwork0.184
R-free0.23920
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4jhw 3h42
RMSD bond length0.021
RMSD bond angle1.893
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 Overall
Low resolution limit [Å]48.191
High resolution limit [Å]3.100
Number of reflections71891
<I/σ(I)>11.96
Completeness [%]100.0
Redundancy11.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP72950.1M Potassium Nitrate, 0.1M Citrate Phosphate pH 4.2, 1 % Tacsimate pH 7.0, 14 (w/v) % PEG 6000

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