5U1T

Crystal structure of the Saccharomyces cerevisiae separase-securin complex at 2.6 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 32 2 1
Unit cell lengths	125.863, 125.863, 271.944
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.667 - 2.600
R-factor	0.2218
Rwork	0.221
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u1s
RMSD bond length	0.011
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MR-Rosetta
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.145	0.062
Rmeas	0.153
Rpim	0.059
Total number of observations	774372
Number of reflections	77491
<I/σ(I)>	4.3
Completeness [%]	100.0	99.9	99.9
Redundancy	10	10	8.2
CC(1/2)		0.998	0.476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	0.25 M Na/K-phosphate (pH 6.0) and 14% (w/v) PEG 3350