5U1T
Crystal structure of the Saccharomyces cerevisiae separase-securin complex at 2.6 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 125.863, 125.863, 271.944 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.667 - 2.600 |
| R-factor | 0.2218 |
| Rwork | 0.221 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u1s |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MR-Rosetta |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.145 | 0.062 | |
| Rmeas | 0.153 | ||
| Rpim | 0.059 | ||
| Total number of observations | 774372 | ||
| Number of reflections | 77491 | ||
| <I/σ(I)> | 4.3 | ||
| Completeness [%] | 100.0 | 99.9 | 99.9 |
| Redundancy | 10 | 10 | 8.2 |
| CC(1/2) | 0.998 | 0.476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 0.25 M Na/K-phosphate (pH 6.0) and 14% (w/v) PEG 3350 |
