5U19

Crystal structure of a methyltransferase involved in the biosynthesis of gentamicin in complex with (1R,2S,3S,4R,6R)-4,6-diamino-3-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-2-hydroxycyclohexyl 2-amino-2-deoxy-alpha-D-glucopyranoside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2016-08-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	65.725, 67.383, 69.571
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.050 - 1.902
R-factor	0.1856
Rwork	0.184
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u4t
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	47.050
High resolution limit [Å]	1.902
Rmerge	0.037
Number of reflections	24922
<I/σ(I)>	22.82
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Calcium chloride, hepes pH 8, peg 6000