5U0C

Structure of Zika virus NS5 RNA polymerase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	120
Detector technology	PIXEL
Collection date	2016-06-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	121.520, 188.710, 192.539
Unit cell angles	90.00, 91.99, 90.00

Refinement procedure

Resolution	89.137 - 3.000
R-factor	0.228
Rwork	0.226
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k6m
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.140	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.144	0.698
Number of reflections	169594
<I/σ(I)>	6
Completeness [%]	98.0	97.9
Redundancy	2.6	2.6
CC(1/2)	0.984	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	277	0.1 M Tris pH 7.5, 0.2 M sodium citrate, and 17% PEG 3350